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2001/2002 Far East MSPPSA report on DNA Sequencing

199 pages, 192 graphs and tables

Published: March 16, 2001

Price: $3395

 

Changes in Far East Sequencing Markets: Future Trends Examined

Do you know the current market share for major products and leading manufacturers in this field? Do you know where the market is headed? This comprehensive report from PhorTech International contains the latest analysis of the DNA Sequencing market in the Far East, including Australasia, Japan, India, and Indonesia. It reveals a serious decline in the market for manual sequencers over the past 3 years, an increasing movement towards auto sequencing, and a strong growth in cycle sequencing over this entire region. Estimating that 36% of life science researchers throughout the Far East are performing DNA sequencing, the market encompasses 16,200 individuals. In this rapidly expanding market, sales of auto sequencers alone amounted to around 200 million dollars in 2000.

This 199 page report results from an extensive web-based survey, designed to probe the attitudes and expectations of a wide cross section of researchers performing DNA sequencing, and provide invaluable feedback on trends in methodology and strengths/weaknesses in current instrumentation. Respondents were characterized by type of organization, source of sequencing data, and the nature of current work. Information was gathered by combining tabular entry check-offs and many open-ended probes. Hundreds of verbatim comments offer invaluable information and suggestions to manufacturers, and feed-back on customer satisfaction. Detailed findings are presented, including 189 graphs and tables, and results compared with PhorTech's recent U.S. survey in this field.

To analyze the installed base of instruments, respondents were asked to identify the types of instruments they had access to; results indicate that 80% have access to several types of instruments; a full breakdown of which is given. The remaining 20% can access only a single instrument. To obtain details regarding these instruments, respondents doing in-lab manual or automated sequencing were asked to itemize each type of sequencer owned by their research group, and provide information regarding brand, model, quantity, date acquired and approximate cost per unit. They were asked why they chose their most recently acquired brand, whether they would choose this unit again, and why. In response to the increase in cycle sequencing, an audit of thermal cyclers used a least one third of the time for cycle sequencing was also included. Interestingly, price did not prove to be a critical consideration amongst customers for either auto sequencers or thermal cyclers.

Respondents were asked to specify the volume of sequence data generated on an annual basis, and the number of reactions run to generate this data from all sources. When asked to provide the percentage of data obtained from in-lab manual sequencing, in-lab automated sequencing, on-site facilities and off-site sequencing services, responses favored the use of core sequencing facilities. More than two thirds of the respondents indicated using a specialized sequencing facility for some or all of their sequencing needs, which is not significantly different from situation in the U.S. market. High volume users are concentrated in the core labs and sequencing facilities, and indeed appear to be the population most likely to make new instrument purchases within the next 12 months. The report goes on to elucidate the most important criteria considered when selecting a service, and the cost per template for this service.

Respondents were asked for an estimate of weekly throughput in terms of number of templates, bases called, and reagent cost per template. The number of manual sequencing gels run in a week was also measured and all sequencing users were asked to estimate the % change in use over the next 12 months.

Turning to methodology, the report examines the throughput for each of five sequencing strategies; mutation analysis, polymorphism screening, expression monitoring, high throughput screening and genetic mapping, together with the different labeling methods employed. All strategies appear to be dominated by reads of >100 bases.

Further questions probed respondents' use of biologicals and reagents. Questions investigated the relative use of kits vs. individual reagents, and the primary suppliers for each. Respondents specified the types and number of templates sequenced weekly for each of seven sequencing methodologies, either in-lab, or by a sequencing service. Current methods of purifying single strand, plasmid and PCR-generated templates were elucidated, together with the methods used for sequencing these templates, and the number of templates per week sequenced using each of 9 different enzymes.

Results revealed a decrease in anticipated use of manual DNA sequencing over the next 12 months, and an overall move towards auto sequencing.

When asked the likelihood of future instrument purchases over the next 12 months, respondents indicated that future interest in purchasing sequence analysis software is twice that for auto sequencers; besides cost considerations, this may reflect wide usage of this tool by all respondents performing sequencing, regardless of the instrument used for analysis. More researchers showed interest in purchasing thermal cyclers than auto sequencers. Most purchases across the board are predicted by researchers working in core labs and sequencing services. Those planning to purchase were asked to specify which suppliers they were considering; results suggest that the main manufacturers will maintain their unit shares.

Additionally, users were asked to select the highest rated manufacturers in key areas, e.g. ease of use, service/support and quality.

Respondents using auto sequencers were also asked to rank manufacturers based on innovation, instrument throughput, running costs and commitment to the field. Finally, respondents were asked to provide verbatim details on instrument features and desired improvements, and on their primary sequencing application and any non-sequencing applications.

PhorTech International has again condensed a vast wealth of information within the pages of this most illuminating report. This comprehensive assessment of the Far East Sequencing Market constitutes required reading for all those hoping to contend in this aggressive and fast-growing market. While providing insight into the competition, and thus an advantage over it, it paints a highly up-to-date picture of the current Far East DNA sequencing market, and outlines trends on which to base smart marketing decisions.

Detailed contents, methodology, demographics, and questionnaire for this reportSee detailed contents, methodology, demographics, and questionnaire for this report in Adobe Acrobat format.